Infestation of cowpea aphid (Aphis craccivora Koch) induced oxidative stress in leaves of soybean (Glycine max (L.) Merr. cv. “Nam Dan”) with a burst in generation of reactive oxygen species (ROS) products such as superoxide anion radical (O2 .-) and hydrogen peroxide (H2O2) recorded around 24 hours after aphid feeding. A continuous increase in content of thiobarbituric acid reactive substances (TBARS) in lipid peroxidation and a defined percentage of injury in aphid-infested leaves (2.20 - 8.79 %) were resulted from the cellular oxidative damage. An enhanced activity of the antioxidant enzymes such as superoxide dismutase (SOD, EC 18.104.22.168) and catalase (CAT, EC 22.214.171.124) was recorded in leaves of soybean “Nam Dan” from 24 to 48 hours post-infestation (hpi). The enhancement of these enzymes function as the antioxidative response that controlled both ROS-accumulation playing as defensive element and ROSdetoxifying, therefore, reduce the oxidative damage within 48-96 hpi. Activity of SOD and CAT also can improve the tolerance of soybean “Nam Dan” to infestation from A. craccivora. Keywords. soybean “Nam Dan”, cowpea aphid, oxidative stress, antioxidative, defense response.
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